Adiponutrin: A multimeric plasma protein

Adiponutrin: a multimeric plasma protein.

The interest in adiponutrin stems from adiponutrin variant I148M, which is strongly associated to non-alcoholic fatty liver disease. Adiponutrin has to date been considered to be solely an intracellular protein, with a role in lipid metabolism in liver and adipose tissue. However, a physiologically relevant role for adiponutrin has not been found. The aim of this study was to investigate the pr...

Solid model representations of a multimeric protein.

placed on the oxidation of choline to betaine via betaine aldehyde which is catalysed by the choline oxidase system. Recent research has indicated that this pathway may indeed be very important for the scavenging of methyl groups from choline 12 I. T h e catabolism of betaine produces one-carbon fragments ;IS ;I result of the sequential demethylation o f betaine to form glycine. T h e first ste...

The evolution of multimeric protein assemblages.

Although the mechanisms by which complex cellular features evolve constitute one of the great unsolved problems of evolutionary biology, it is clear that the emergence of new protein-protein interactions, often accompanied by the diversification of duplicate genes, is involved. Using information on the levels of protein multimerization in major phylogenetic groups as a guide to the patterns tha...

Recombination-stable multimeric green fluorescent protein

Rapid assembly of a multimeric membrane protein pore.

We have observed the assembly of the staphylococcal pore-forming toxin α-hemolysin using single-molecule fluorescence imaging. Surprisingly, assembly from the monomer to the complete heptamer is extremely rapid, occurring in <5 ms. No lower order oligomeric intermediates are detected. Monte Carlo simulation of our experiment shows that assembly is diffusion limited, and pore formation is depend...